Abstract brevetto
A protein comprising a single or a combination of three intracytoplasmatic peptide stretches of the C-terminal domain of the Na+,K+-ATPase alpha sub-unit is herein disclosed, all said peptide stretches binding HIV-1 Tat protein. Said protein is the fusion of said three stretches, corresponding to the 824-843, 939-951 e 1007-1023 regions of said domain and have the sequence NH2-YEQAESDIMKRQPRNPKTDK-COOH, NH2-TRRNSVFQQGMKN-COOH, NH2-LIIRRRPGGWVEKETYY-COOH. The invention also discloses a mammal expression vector ex pressing said protein and the corresponding DNA. The use of said substances is also disclosed for inhibiting HIV-1 Tat protein transactivation, inhibiting HIV-1 replication and treating HIV-1 infection and related diseases